Figure 3.

Western immunoblot (top, male fetal hearts) and analysis (bottom) of fusion proteins (Mfn-2 and OPA-1) in isolated cardiac mitochondria of left ventricles. Male and female fetuses were exposed to normoxia (NMX, circles) and early-onset [25 days (25d) gestation, 39d duration] and late-onset (50d gestation, 14 duration) hypoxia (HPX, triangles). Protein expression was measured in fetal heart ventricles obtained at term (64d gestation). Density values are ratios of target band values normalized to VDAC as a loading control. *Significant differences from NMX controls; n = 7 animals in each group (*P < 0.05, **P < 0.01, ****P < 0.0001). VDAC, voltage-dependent anion channel.