Skip to main content
. 2021 Dec 15;12:804870. doi: 10.3389/fimmu.2021.804870

Figure 1.

Figure 1

Effects of early weaning on colonic morphology and mucosal barrier function. (A) Representative images of hematoxylin and eosin-stained colon sections from SP and WP groups (scale bars, 50 μm). (B) Statistical analysis of crypt depth. (C) Stacked bar graph showing the total histology scores and individual histological criteria score differences between the two groups. (D) Representative images of periodic acid-Schiff-alcian blue (PAS-AB)-stained colonic sections from the SP and WP groups (scale bars, 50 μm). (E) Mucin layer width in the colon of SPs and WPs was quantified from images of AB staining. (F) Statistical analysis of the numbers of mucus-producing goblet cells. (G) Immunofluorescence staining for the goblet cell marker MUC2 (green). Scale bars, 50 μm. (H, I) Western blotting results for ZO-1, occludin, and claudin-3 expression in the colon. All data are presented as means ± SEM, Student’s t-test, *P < 0.05, **P < 0.01; n = 6. SP, suckling piglet; WP, weaning piglets.