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. 2021 Dec 15;8:763851. doi: 10.3389/fcvm.2021.763851

Figure 2.

Figure 2

Design of guide RNA and identification primer. (A) Guide #5 TGATGCAATGGTCAGAGATGTGG; (B) the sequencing result of the activity verification; (C) the optimized amino acid sequence alignment (the lowercase font is the optimized base).