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Results of pathology, western blot, and hybridization experiments. (A) TU zebrafish; (B) F1 dhfr heterozygous self-progeny (the red arrow points to the heart area). (C,D) WT, dhfr KI heterozygote, and dhfr KI homozygous DHFR protein expression analysis (GAPDH was used as an internal control). E: impaired fertility in zebrafish knocked in by dhfr (*P < 0.05, ***P < 0.001, ****P < 0.0001).
