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. 2021 Dec 15;12:767333. doi: 10.3389/fphar.2021.767333

TABLE 1.

Real-time RT-PCR oligonucleotide primers.

Gene Primer Sequence (5′-3′) PCR product (bp)
β-actin Forward TGT​TAC​CAA​CTG​GGA​CGA​CA 165
(NM_007393.3) Reverse GGG​GTG​TTG​AAG​GTC​TCA​AA
TLR4 Forward GAG​CCG​GAA​AGT​TAT​TGT​GG 150
(NM_019178.2) Reverse AGC​AAG​GAC​TTC​TCC​ACT​TTC​T
Occludin Forward GAG​GGT​ACA​CAG​ACC​CCA​GA 161
(NM_031329.3) Reverse CAG​GAT​TGC​GCT​GAC​TAT​GA
Claudin-1 Forward CCT​CCA​ATG​CCG​TTC​TGT​AT 118
(NM_031699.3) Reverse AGG​GCC​TTT​GCT​ACA​GAT​GA