REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-alpha smooth muscle actin (ACTA)	Abcam	ab125044
Mouse monoclonal anti-CD22	Leica	PA0249
Mouse monoclonal anti-CD3	Leica	PA0553
Mouse monoclonal anti-CD34	Leica	PA0212
Rabbit monoclonal anti-CDX2	Abcam	ab76541
Rabbit monoclonal anti-Chromogranin A	Abcam	ab68271
Rabbit monoclonal anti-FOXA2	Abcam	ab108422
Mouse monoclonal anti-Glucagon	Sigma	G2654
Rabbit monoclonal anti-Insulin	Cell Signaling Technology	3014S
Goat polyclonal anti-NKX6-1	LSBio	LS-C124275
Critical commercial assays
C-Peptide	ADVIA Centaur and ADVIA Centaur XP systems.	03649928
Experimental models: Cell lines
Endoderm cells	Schulz et al., 2012	PEC-01, pancreatic
Human embryonic stem cells	Ethically consented embryo, NIH Registration Number 0041	CyT49
Software and algorithms
Image analysis software	Definiens	Tissue Studio