REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
InVivoPlus anti-mouse 4-1BB (CD137)	BioXCell	CAT# BP0239, RRID:AB_2894814
InVivoPlus anti-mouse PD-1 (CD279)	BioXCell	CAT#: BP0146, RRID:AB_2894808
InVivoPlus anti-mouse CTLA-4 (CD152)	BioXCell	CAT#: BP0164, RRID:AB_2894809
InVivoPlus anti-mouse LAG-3	BioXCell	CAT#: BP0174, RRID:AB_2894812
InVivoPlus anti-mouse TIM-3 (CD366)	BioXCell	CAT#: BP0115, RRID:AB_2894805
InVivoPlus anti-mouse CD4	BioXCell	CAT#: BP0003-1, RRID:AB_2891358
InVivoPlus anti-mouse CD8α	BioXCell	CAT#: BP0061, RRID:AB_2891359
InVivoPlus anti-mouse NK1.1	BioXCell	CAT#: BE0036, RRID:AB_1107737
Recombinant Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody	Abcam	CAT#: ab210438, RRID:AB_2895210
Glut4 Antibody [Alexa Fluor® 647]	Novus Biologicals	Cat#: NBP1-49533AF647, RRID:AB_2895213
Anti-Ovalbumin antibody	Abcam	Cat#: ab186717, RRID:AB_2895212
Anti-Proteasome 20S LMP7 antibody	Abcam	Cat#: ab3329, RRID:AB_303708
FoxO4 Antibody	Cell Signaling Technology	Cat#: 9472, RRID:AB_10831833
HRP Anti-beta Actin antibody [AC-15]	Abcam	Cat#: ab49900, RRID:AB_867494
Recombinant Anti-PSMB10/MECL1 antibody	Abcam	Cat#: ab183506, RRID:AB_2895211
Recombinant Anti-Ubiquitin (linkage-specific K48) antibody [EP8589]	Abcam	Cat#: ab140601, RRID:AB_2783797
PE Rat Anti-Mouse CD105	BD Biosciences	CAT#: 562759, RRID:AB_2737774
PE-Cy7 Rat Anti-Mouse CD8a	BD Biosciences	CAT#: 552877, RRID:AB_394506
APC Mouse Anti-Rat CD90/Mouse CD90.1	BD Biosciences	CAT#: 561409, RRID:AB_10683163
APC Rat Anti-Mouse CD274	BD Biosciences	Cat#: 564715, RRID:AB_2687479
APC Rat Anti-Mouse CD44	BD Biosciences	Cat#: 559250, RRID:AB_398661
APC-Cy7 Rat Anti-Mouse CD45	BD Biosciences	Cat#: 557659, RRID:AB_396774
BV421 Mouse Anti-Mouse H-2K[b]	BD Biosciences	Cat#: 562942, RRID:AB_2737908
PE Hamster Anti-Mouse CD80	BD Biosciences	Cat#: 553769, RRID:AB_395039
PE Mouse Anti-Mouse H-2D[b]	BD Biosciences	Cat#: 553574, RRID:AB_394932
PE Rat Anti-Mouse CD73	BD Biosciences	Cat#: 550741, RRID:AB_393860
PE Rat Anti-Mouse CD86	BD Biosciences	Cat#: 5536922, RRID:AB_394994
PE anti-mouse H-2Kb bound to SIINFEKL Antibody	BioLegend	Cat# 141604, 141603, RRID:AB_10895905, RRID:AB_10897938
FoxO1 (C29H4) Rabbit mAb	Cell Signaling Technology	Cat#: 2880s, RRID:AB_2106495
HIF-1α Antibody	Cell Signaling Technology	Cat #: 3716, RRID:AB_2116962
K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb	Cell Signaling Technology	Cat#: 5621s, RRID:AB_10827985
p38 MAPK Antibody	Cell Signaling Technology	Cat#: 9212s, RRID:AB_330713
P-Akt (Ser473) Antibody	Cell signaling Technology	Cat#: #9271, RRID:AB_329825
Phospho-FoxO1 (Ser256) Antibody	Cell signaling Technology	Cat#: 9461, RRID:AB_329831
P-p38 MAPK (Thr180/Tyr182) Antibody	Cell signaling Technology	Cat#: 4511, RRID:AB_2139682
Chemicals, peptides, and recombinant proteins
Methylmalonate	Cayman Chemical	Item No. 14885
SIINFEKLE	GenScript	N/A
Chloroquine	InvivoGen	Cat#: tlrl-chq
CLODRONATE LIPOSOMES & CONTROL LIPOSOMES (PBS)	LIPOSOMA	CSKU: CP-005-005
Recombinant Murine GM-CSF	PeproTech	Cat#: 315-03
Recombinant Murine IFN-γ	PeproTech	Cat#: 315-05
XenoLight D-Luciferin - K+ Salt Bioluminescent Substrate	PerkinElmer	Cat#: 122799
2-Deoxy-D-glucose	Sigma-Aldrich	Cat#: D8375-1G
Accutase® solution	Sigma-Aldrich	Cat#: A6964
Albumin from chicken egg white	Sigma-Aldrich	Cat#: A5503
Alizarin Red S	Sigma-Aldrich	Cat#: A5533-25G
Lipopolysaccharides from Escherichia coli O111:B4	Sigma-Aldrich	Cat#: L2630-10MG
Oil Red O	Sigma-Aldrich	Cat#: O0625-25G
CellTrace Violet Cell Proliferation Kit, for flow cytometry	ThermoFisher Scientific	Cat#: C34557
DQ ovalbumin	ThermoFisher Scientific	Cat#: D12053
Lucifer Yellow CH	ThermoFisher Scientific	Cat#: L1177
MitoSOX Red Mitochondrial Superoxide Indicator, for live-cell imaging	ThermoFisher Scientific	Cat#: M36008
MitoTracker Deep Red FM	ThermoFisher Scientific	Cat#: M22426
MitoTracker Green FM	ThermoFisher Scientific	Cat#: M7514
Ovalbumin, Alexa Fluor 647 Conjugate	ThermoFisher Scientific	Cat#: O34784
pHrodo Green Dextran, 10,000 MW, for Endocytosis	ThermoFisher Scientific	Cat# P35368
pHrodo Red AM Intracellular pH Indicator	ThermoFisher Scientific	Cat#: P35372
FTY720	Tocris	N/A
Critical commercial assays
RNeasy Mini Kit (50)	QIAGEN	Cat#: 74104
Mouse IFN-gamma DuoSet ELISA	R&D systems	Cat#: DY485-05
Mouse IL-12 p70 Quantikine ELISA Kit	R&D systems	Cat#: M1270
Mouse IL-6 Quantikine ELISA Kit	R&D systems	Cat#: M6000B
Mouse Chemokine Array C1	RayBiotech	Cat#: AAM-CHE-1-2
EasySep Mouse CD8a Positive Selection Kit	StemCell Technologies	Cat #: 18753
EasySep Mouse CD8a Positive Selection Kit II	StemCell Technologies	Cat#:18953
Deposited data
RNA-seq data for ctl MSCs and MSC-IPr	GEO	GEO: GSE183055
Experimental models: Cell lines
Mouse: A20	ATCC	CAT# TIB-208, RRID:CVCL1940
Mouse: B16F0	ATCC	ATCC Cat# CRL-6322, RRID:CVCL0604
Mouse: E.G7-OVA [derivative of EL4]	ATCC	ATCC Cat# CRL-2113, RRID:CVCL3505
Mouse: EL4	ATCC	Cat# TIB-39, RRID:CVCL0255
293-GP2	Clontech	RRID:CVCLWI48
Experimental models: Organisms/strains
Mouse: BALB/cAnCrl	Charles River	Strain code: 028
Mouse: C57BL/6NCrl	Charles River	Strain code: 027
Mouse: OT1 (C57BL/6-Tg(TcraTcrb)1100Mjb/J)	The Jackson Laboratory	Strain code: 003831
Mouse: Batf3-deficient mouse (B6.129S(C)-Batf3tm1Kmm/J)	The Jackson Laboratory	Strain code: 013755
Mouse: MHCI-deficient mouse (B6.129P2-H2-K1tm1Bpe H2-D1tm1Bpe/DcrJ)	The Jackson Laboratory	Strain code: 019995
Recombinant DNA
Murine IPr construct	This paper	n/a
Software and algorithms
FlowJo v10	FlowJo	https://www.flowjo.com/solutions/flowjo/downloads
Prism-GraphPad	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Software used for statistical analysis	GraphPad Software	https://www.graphpad.com/scientific-software/prism/