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. 2021 Dec 15;15:774305. doi: 10.3389/fncel.2021.774305

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Copyright © 2021 Zhou, Xu, Liao, Tang, Li and Hou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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YTHDC1 depletion facilitates BV2 microglia M1 activation and migration in vitro. (A–C) The transfection efficiency of YTHDC1 knockdown in BV2 cells was measured by qRT-PCR (A) and Western blotting (B,C). (D,E) Western blot analysis of iNOS, COX2 and TNF-α in the different groups. (F) Immunofluorescence analysis of iNOS in the different groups. (G,H) Wound healing assays in the different groups. (I,J) Transwell assays in the different groups. NC: cells transfected with negative control shRNA; ShYTHDC1 (1, 2, and 3) or ShYTHDC1: cells transfected with YTHDC1 shRNA. *P < 0.05, ^**P < 0.01, and ^***P < 0.001 by Student’s t-test. The data are presented as the mean ± SD. (n = 3). Scale bar, 200 μm.