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. 2021 Nov 29;27:256–275. doi: 10.1016/j.omtn.2021.11.019

Figure 9.

Figure 9

miR-148a-3p overexpression or PTEN silencing protects miR-148a-3p/p35/PTEN signaling and PTEN/Akt/CREB feedforward in AD mice

(A–D) Co-localization of p35 (A and B) and PTEN (C and D) with phosphorylated tau PHF-1 in the hippocampus (A and C) and cortex (B and D) of APP/PS1 mice treated with miR-148a-3p mimics, PTEN siRNAs, and scrambled controls. Scale bar, 50 μm. (E and F) Representative western blot images of pTS396, pTS404, pTS199, Tau, p35, p25, CDK5, p-PTEN, and PTEN (E) and quantification of ratios of S396/Tau, S404/Tau, S199/Tau, and p-PTEN/PTEN and expression of p25, p35, and CDK5 (F) in the hippocampus of APP/PS1 mice treated with miR-148a-3p mimic and scrambled control. (G and H) Representative western blot images of pTS396, pTS404, pTS199, Tau, p-PTEN, PTEN, p-Akt, Akt, p-CREB, and CREB (G) and quantification of ratios of S396/Tau, S404/Tau, S199/Tau, p-PTEN/PTEN, p-Akt/Akt, and p-CREB/CREB (H) in the hippocampus of APP/PS1 mice treated with PTEN siRNA and scrambled control. (I) miR-148a-3p expression in the hippocampus of APP/PS1 mice treated with PTEN siRNA and scrambled control. Results represent means ± SEM, n = 5. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 versus WT mice. #p < 0.05, ##p < 0.01, ###p < 0.001 versus APP/PS1 scrambled controls. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.