Table 2.
Primary and secondary antibodies used in western blot analysis
| Primary antibody | Dilution | Source | Secondary antibody |
|---|---|---|---|
| Anti- tau (phospho S202 + T205) rabbit mAb | 1:1,000 | Abcam | HRP-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (1:10,000, EarthOx) |
| Anti-tau (phospho S404) rabbit mAb | 1:1,000 | Abcam | |
| Anti-tau (phospho S396) rabbit mAb | 1:1,000 | Abcam | |
| Anti-tau (phospho S199) rabbit mAb | 1:5,000 | Abcam | |
| Anti-Tau rabbit mAb | 1:2,000 | Abcam | |
| Anti-p35 rabbit pAb | 1:500 | Abcam | |
| Anti-CDK5 rabbit mAb | 1:2,000 | Abcam | |
| Anti-PTEN (phospho S380) rabbit mAb | 1:1,000 | Abcam | |
| Anti-PTEN rabbit mAb | 1:1,000 | Abcam | |
| Anti-Akt (phospho S473) rabbit mAb | 1:5,000 | Abcam | |
| Anti-Akt rabbit mAb | 1:1,000 | CST | |
| Anti-CREB (phospho S133) rabbit mAb | 1:5,000 | Abcam | |
| Anti-CREB rabbit mAb | 1:500 | Abcam | |
| Anti-p38 MAPK (phospho T180/Y182) rabbit mAb | 1:1,000 | CST | |
| Anti-p38 MAPK rabbit mAb | 1:1,000 | Abcam | |
| Anti-p44/42 MAPK (ERK1/2) (phospho T202/Y204) mouse mAb | 1:2,000 | CST | |
| Anti-p44/42 MAPK (ERK1/2) rabbit mAb | 1:1,000 | CST | |
| Anti-GSK-3β (phospho S9) rabbit mAb | 1:1,000 | Abcam | |
| Anti-GSK-3β rabbit mAb | 1:1,000 | CST | |
| Anti-APP rabbit mAb | 1:1,000 | CST | |
| Anti-GAPDH rabbit mAb | 1:5,000 | Proteintech | |
| Anti-α-tubulin rabbit pAb | 1:5,000 | Proteintech | |
| Anti-β-actin mouse mAb | 1:5,000 | Proteintech |
Abcam, Cambridge, MA, USA; CST, Cell Signaling Technology, Danvers, MA, USA; Proteintech, Rosemont, IL, USA; EarthOx Life Sciences, Millbrae, CA, USA.