Figure 7.

Validation of randomly selected DEGs. (A) Validation of 10 significant DEGs between healthy and acutely infected, dead pigs (H vs. A) by qPCR. (B) Validation of 10 significant DEGs between healthy and cohabiting asymptomatic pigs (H vs. C) by RT-qPCR using the log₂ (fold Change) method. (C) Validation of KEGG pathway enrichment 20 genes between healthy and acutely infected, dead pigs (H vs. A) by qPCR. (D) Validation of KEGG pathway enrichment 20 genes between healthy and cohabiting asymptomatic pigs (H vs. C) by RT-qPCR using the log₂ (fold Change) method. The RNA expression of each target gene was normalized to GAPDH expression. Three biological replicates were set up for each sample.