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. 2021 Dec 15;8:759690. doi: 10.3389/fnut.2021.759690

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Copyright © 2021 Hafner, Mulcahy, Carlson, Hartley, Sun, Westerhoff, Qi, Bridges and Gregg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Offspring liver morphology and metabolic characteristics. Liver weights at necropsy (6 months of age) in male (A) and female (B) offspring during both ND feeding (no HFD) and HFD rechallenge. ND results are white for Ctrl PN and blue for HFD PN. HFD rechallenge results are in light gray for Ctrl PN + HFD and dark gray for HFD PN + HFD (C). Liver triglyceride quantification and representative histology by hematoxylin and eosin (H&E) staining to visualize fat accumulation as open spaces. (D) Liver fibrosis quantification by picro-sirius staining with representative images showing red-stained collagen fibers. Ctrl, control; HFD, high-fat diet; PN, post-natal; HPF, high-power field. *p < 0.05, **p < 0.01, ****p < 0.0001.