Figure 5.

CPT1A and ACACB expression levels are associated with carboplatin resistance in 2 of the 3 cell line pairs, and pharmacologic inhibition of CPT1A sensitizes cells to carboplatin

(A) CPT1A protein abundance is higher in PEA2^R and PEO4^R, although acetyl-CoA carboxylase 2 (ACACB) protein abundance is lower in PEA2^R and PEO4^R. Western blot data (see image and bar graphs) confirm the LC-MS/MS results.

(B–D) Perhexiline sensitizes HGSOC cell lines to carboplatin.

(E) Perhexiline sensitizes non-tumorigenic FT4 cells to carboplatin.

Data in (B)–(E) are an average of 3 biological repeats each with 3 technical repeats. p values (Student’s t test) are provided.