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. 2021 Dec 15;12:742020. doi: 10.3389/fmicb.2021.742020

TABLE 2.

The amount of carbohydrates, organic acids, and alcohols for Sc fermentation.

Time Batch A Batch B Batch C
Fructose 0 8.85 ± 0.08** 5.64 ± 0.06** 0.73 ± 0.02**
4 9.15 ± 0.08** 5.34 ± 0.09** 0.89 ± 0.09**
8 9.69 ± 0.06** 5.39 ± 0.08** 0.84 ± 0.05**
12 8.76 ± 0.10** 4.86 ± 0.09** 0.72 ± 0.02**
24 0.81 ± 0.02** 0.28 ± 0.06** N.D.
48 0.18 ± 0.03** N.D. N.D.
72 0.05 ± 0.01** N.D. N.D.
Glucose 0 3.27 ± 0.17** 2.26 ± 0.06** 0.46 ± 0.07**
4 3.33 ± 0.06** 2.06 ± 0.06** 0.54 ± 0.04**
8 3.44 ± 0.06** 1.94 ± 0.05** 0.43 ± 0.02**
12 2.86 ± 0.06** 1.19 ± 0.07** 0.22 ± 0.03**
24–72 N.D. N.D. N.D.
Maltose 0 1.25 ± 0.03** 0.85 ± 0.04* 0.67 ± 0.07**
4 1.48 ± 0.09** 1.16 ± 0.05** 1.09 ± 0.07NS(0.574)
8 1.42 ± 0.06NS(0.326) 1.36 ± 0.04** 1.16 ± 0.03**
12 1.53 ± 0.10NS(0.626) 1.33 ± 0.06NS(0.899) 1.48 ± 0.43NS(0.756)
24 0.32 ± 0.01** 0.23 ± 0.02** 0.20 ± 0.01NS(0.110)
48 0.17 ± 0.01** 0.10 ± 0.01** 0.11 ± 0.01NS(0.688)
72 0.09 ± 0.01NS(0.783) 0.095 ± 0.002* 0.13 ± 0.01*
Citric acid 0 0.09 ± 0.01** 0.052 ± 0.001** 0.038 ± 0.001**
4 0.093 ± 0.003** 0.056 ± 0.003** 0.049 ± 0.002*
8 0.09 ± 0.01** 0.053 ± 0.008** 0.043 ± 0.006NS(0.408)
12 0.09 ± 0.01* 0.054 ± 0.002** 0.052 ± 0.007NS(0.899)
24 0.09 ± 0.01** 0.046 ± 0.001** 0.044 ± 0.002NS(0.899)
48 0.052 ± 0.002* 0.032 ± 0.002NS(0.055) 0.032 ± 0.001NS(0.867)
72 0.05 ± 0.01* 0.021 ± 0.001* 0.037 ± 0.001NS(0.782)
Lactic acid 0–12 N.D. N.D. N.D.
24 0.063 ± 0.003** 0.101 ± 0.002** 0.131 ± 0.003*
48 0.20 ± 0.01** 0.27 ± 0.02** 0.14 ± 0.01**
72 0.28 ± 0.01** 0.37 ± 0.02NS(0.090) 0.24 ± 0.01**
Acetic acid 0–12 N.D. N.D. N.D.
24 N.D. 0.08 ± 0.01** 0.29 ± 0.02**
48 0.12 ± 0.02** 0.30 ± 0.02** 0.30 ± 0.01NS(0.852)
72 0.23 ± 0.01NS(0.899) 0.23 ± 0.01* 0.28 ± 0.02*
Mannitol 0–12 N.D. N.D. N.D.
24 0.161 ± 0.001** 0.084 ± 0.001** N.D.
48 0.26 ± 0.01** 0.18 ± 0.01** N.D.
72 0.37 ± 0.04** 0.183 ± 0.001** N.D.
Erythritol 0–12 N.D. N.D. N.D.
24 0.05 ± 0.01NS(0.483) 0.04 ± 0.01NS(0.702) 0.062 ± 0.001NS(0.184)
48 0.095 ± 0.003* 0.06 ± 0.01NS(0.265) 0.071 ± 0.02NS(0.350)
72 0.10 ± 0.01** 0.07 ± 0.01NS(0.237) 0.092 ± 0.004NS(0.068)
Glycerol 0 0.047 ± 0.002NS(0.324) 0.034 ± 0.001** N.D.
4 0.06 ± 0.05NS(0.769) 0.04 ± 0.03NS(0.198) N.D.
8 0.06 ± 0.06NS(0.899) 0.05 ± 0.04NS(0.281) N.D.
12 0.064 ± 0.001NS(0.651) 0.053 ± 0.003** 0.019 ± 0.001**
24 0.22 ± 0.01** 0.12 ± 0.01** 0.041 ± 0.002**
48 0.21 ± 0.01** 0.142 ± 0.002** 0.048 ± 0.006**
72 0.22 ± 0.01** 0.13 ± 0.01** 0.014 ± 0.001**
Ethanol 0 0.85 ± 0.07** 1.26 ± 0.06* 0.67 ± 0.05**
4 1.17 ± 0.07* 1.47 ± 0.11NS(0.510) 1.08 ± 0.09**
8 1.37 ± 0.03NS(0.686) 1.45 ± 0.18NS(0.149) 1.17 ± 0.06NS(0.051)
12 1.34 ± 0.04* 1.56 ± 0.09NS(0.127) 1.22 ± 0.05**
24 0.23 ± 0.01** 0.32 ± 0.01NS(0.136) 0.20 ± 0.02**
48 0.17 ± 0.01** 0.10 ± 0.01* 0.13 ± 0.01NS(0.060)
72 0.09 ± 0.01NS(0.625) 0.10 ± 0.01* 0.13 ± 0.01*
1,3-propanediol 0–72 N.D. N.D. N.D.

The carbohydrates, organic acids, and alcohols were expressed as grams of compounds identified per liter of extract (g/l). The experiments were made in triplicate, and the values represent the average and standard deviation of them. Data normality was studied using the Shapiro–Wilk test. For values, p > 0.05, the data are normally distributed, and in the descriptive statistics table, we passed mean ± SD (n = 3). One-way ANOVA with post hoc Tukey HSD was used to determine if there were significant differences between batch A, batch B, and batch C for each compound. The p < 0.05 value corresponding to the F-statistic of one-way ANOVA was obtained, the calculations were continued, and the significance of the differences for groups of two by two substrates was obtained. In brackets, Tukey HSD p-value and Tukey HSD inference were passed as follows: on the first column, batch A vs. batch B; on the second column, batch B vs. batch C; on the third column, batch A vs. batch C.

The symbols were used as follows: **p < 0.01, *p < 0.05, NS(insignifiant) p > 0.05. N.D., not detected.