TABLE 2.
The amount of carbohydrates, organic acids, and alcohols for Sc fermentation.
| Time | Batch A | Batch B | Batch C | |
| Fructose | 0 | 8.85 ± 0.08** | 5.64 ± 0.06** | 0.73 ± 0.02** |
| 4 | 9.15 ± 0.08** | 5.34 ± 0.09** | 0.89 ± 0.09** | |
| 8 | 9.69 ± 0.06** | 5.39 ± 0.08** | 0.84 ± 0.05** | |
| 12 | 8.76 ± 0.10** | 4.86 ± 0.09** | 0.72 ± 0.02** | |
| 24 | 0.81 ± 0.02** | 0.28 ± 0.06** | N.D. | |
| 48 | 0.18 ± 0.03** | N.D. | N.D. | |
| 72 | 0.05 ± 0.01** | N.D. | N.D. | |
| Glucose | 0 | 3.27 ± 0.17** | 2.26 ± 0.06** | 0.46 ± 0.07** |
| 4 | 3.33 ± 0.06** | 2.06 ± 0.06** | 0.54 ± 0.04** | |
| 8 | 3.44 ± 0.06** | 1.94 ± 0.05** | 0.43 ± 0.02** | |
| 12 | 2.86 ± 0.06** | 1.19 ± 0.07** | 0.22 ± 0.03** | |
| 24–72 | N.D. | N.D. | N.D. | |
| Maltose | 0 | 1.25 ± 0.03** | 0.85 ± 0.04* | 0.67 ± 0.07** |
| 4 | 1.48 ± 0.09** | 1.16 ± 0.05** | 1.09 ± 0.07NS(0.574) | |
| 8 | 1.42 ± 0.06NS(0.326) | 1.36 ± 0.04** | 1.16 ± 0.03** | |
| 12 | 1.53 ± 0.10NS(0.626) | 1.33 ± 0.06NS(0.899) | 1.48 ± 0.43NS(0.756) | |
| 24 | 0.32 ± 0.01** | 0.23 ± 0.02** | 0.20 ± 0.01NS(0.110) | |
| 48 | 0.17 ± 0.01** | 0.10 ± 0.01** | 0.11 ± 0.01NS(0.688) | |
| 72 | 0.09 ± 0.01NS(0.783) | 0.095 ± 0.002* | 0.13 ± 0.01* | |
| Citric acid | 0 | 0.09 ± 0.01** | 0.052 ± 0.001** | 0.038 ± 0.001** |
| 4 | 0.093 ± 0.003** | 0.056 ± 0.003** | 0.049 ± 0.002* | |
| 8 | 0.09 ± 0.01** | 0.053 ± 0.008** | 0.043 ± 0.006NS(0.408) | |
| 12 | 0.09 ± 0.01* | 0.054 ± 0.002** | 0.052 ± 0.007NS(0.899) | |
| 24 | 0.09 ± 0.01** | 0.046 ± 0.001** | 0.044 ± 0.002NS(0.899) | |
| 48 | 0.052 ± 0.002* | 0.032 ± 0.002NS(0.055) | 0.032 ± 0.001NS(0.867) | |
| 72 | 0.05 ± 0.01* | 0.021 ± 0.001* | 0.037 ± 0.001NS(0.782) | |
| Lactic acid | 0–12 | N.D. | N.D. | N.D. |
| 24 | 0.063 ± 0.003** | 0.101 ± 0.002** | 0.131 ± 0.003* | |
| 48 | 0.20 ± 0.01** | 0.27 ± 0.02** | 0.14 ± 0.01** | |
| 72 | 0.28 ± 0.01** | 0.37 ± 0.02NS(0.090) | 0.24 ± 0.01** | |
| Acetic acid | 0–12 | N.D. | N.D. | N.D. |
| 24 | N.D. | 0.08 ± 0.01** | 0.29 ± 0.02** | |
| 48 | 0.12 ± 0.02** | 0.30 ± 0.02** | 0.30 ± 0.01NS(0.852) | |
| 72 | 0.23 ± 0.01NS(0.899) | 0.23 ± 0.01* | 0.28 ± 0.02* | |
| Mannitol | 0–12 | N.D. | N.D. | N.D. |
| 24 | 0.161 ± 0.001** | 0.084 ± 0.001** | N.D. | |
| 48 | 0.26 ± 0.01** | 0.18 ± 0.01** | N.D. | |
| 72 | 0.37 ± 0.04** | 0.183 ± 0.001** | N.D. | |
| Erythritol | 0–12 | N.D. | N.D. | N.D. |
| 24 | 0.05 ± 0.01NS(0.483) | 0.04 ± 0.01NS(0.702) | 0.062 ± 0.001NS(0.184) | |
| 48 | 0.095 ± 0.003* | 0.06 ± 0.01NS(0.265) | 0.071 ± 0.02NS(0.350) | |
| 72 | 0.10 ± 0.01** | 0.07 ± 0.01NS(0.237) | 0.092 ± 0.004NS(0.068) | |
| Glycerol | 0 | 0.047 ± 0.002NS(0.324) | 0.034 ± 0.001** | N.D. |
| 4 | 0.06 ± 0.05NS(0.769) | 0.04 ± 0.03NS(0.198) | N.D. | |
| 8 | 0.06 ± 0.06NS(0.899) | 0.05 ± 0.04NS(0.281) | N.D. | |
| 12 | 0.064 ± 0.001NS(0.651) | 0.053 ± 0.003** | 0.019 ± 0.001** | |
| 24 | 0.22 ± 0.01** | 0.12 ± 0.01** | 0.041 ± 0.002** | |
| 48 | 0.21 ± 0.01** | 0.142 ± 0.002** | 0.048 ± 0.006** | |
| 72 | 0.22 ± 0.01** | 0.13 ± 0.01** | 0.014 ± 0.001** | |
| Ethanol | 0 | 0.85 ± 0.07** | 1.26 ± 0.06* | 0.67 ± 0.05** |
| 4 | 1.17 ± 0.07* | 1.47 ± 0.11NS(0.510) | 1.08 ± 0.09** | |
| 8 | 1.37 ± 0.03NS(0.686) | 1.45 ± 0.18NS(0.149) | 1.17 ± 0.06NS(0.051) | |
| 12 | 1.34 ± 0.04* | 1.56 ± 0.09NS(0.127) | 1.22 ± 0.05** | |
| 24 | 0.23 ± 0.01** | 0.32 ± 0.01NS(0.136) | 0.20 ± 0.02** | |
| 48 | 0.17 ± 0.01** | 0.10 ± 0.01* | 0.13 ± 0.01NS(0.060) | |
| 72 | 0.09 ± 0.01NS(0.625) | 0.10 ± 0.01* | 0.13 ± 0.01* | |
| 1,3-propanediol | 0–72 | N.D. | N.D. | N.D. |
The carbohydrates, organic acids, and alcohols were expressed as grams of compounds identified per liter of extract (g/l). The experiments were made in triplicate, and the values represent the average and standard deviation of them. Data normality was studied using the Shapiro–Wilk test. For values, p > 0.05, the data are normally distributed, and in the descriptive statistics table, we passed mean ± SD (n = 3). One-way ANOVA with post hoc Tukey HSD was used to determine if there were significant differences between batch A, batch B, and batch C for each compound. The p < 0.05 value corresponding to the F-statistic of one-way ANOVA was obtained, the calculations were continued, and the significance of the differences for groups of two by two substrates was obtained. In brackets, Tukey HSD p-value and Tukey HSD inference were passed as follows: on the first column, batch A vs. batch B; on the second column, batch B vs. batch C; on the third column, batch A vs. batch C.
The symbols were used as follows: **p < 0.01, *p < 0.05, NS(insignifiant) p > 0.05. N.D., not detected.