FIGURE 3.

Characterization of the photosynthetic solution. Different densities of C. reinhardtii were added to a standard solution for organ preservation (RLM). The oxygen production rate of microalgae was maintained after 24 h, except for 10⁶ C. reinhardtii/ml, which increased (A). Up to 10⁸ C. reinhardtii/ml, no significant differences were observed in the osmolality (B) and rheological properties of the solution (C). Zebrafish larvae were exposed for 24 h to photosynthetic solution containing different densities of microalgae, in the dark. Up to 10⁸ C. reinhardtii/ml larvae presented normal phenotypes compared to control [E3; (D)]. Mild and severe mortality were observed at 10⁸ and 10⁹ C. reinhardtii/ml, respectively (E). Scale bars represent 1 mm in D. Data are expressed as mean ± SD; N = 3, 4; *p < 0.05, ***p ≤ 0.001 (one-way ANOVA followed by Tukey’s test in A; one-way ANOVA followed by Dunnett’s test in B; two-way ANOVA followed by Sidak´s test in C); different letters in E indicate significant differences with p < 0.05 (one-way ANOVA followed by Tukey´s test).