Figure 2.

Cell death is exacerbated when lymphangiogenesis is blocked in cisplatin (Cp) nephrotoxicity. A: representative images of TUNEL staining on transverse kidney sections at day 3 after Cp administration. Whole kidney sections were imaged, and TUNEL-positive cells were quantitated in the cortex (B) and medulla (C). D: protein lysates from whole kidney tissue were analyzed for cleaved caspase-3 (CC3) expression. Anti-β-actin was used as a loading control. Densitometric values were calculated and expressed in arbitrary units (AU). Data are expressed as means ± SE; n = 6–17 animals per group. *P < 0.05 vs. vehicle control; #P < 0.05 vs. Cp + DMSO using one-way ANOVA followed by a Tukey’s multiple comparisons test. Scale bar = 100 µm.