Figure 5.

The autofluorescence imaging signature reveals erythrocyte-associated vascular congestion in cisplatin (Cp)-treated animals without MAZ51 treatment. Maximum projection images of three-dimensional volume of autofluorescence of Cp + vehicle-treated (A) and Cp + MAZ51-treated (B) whole kidney sections (left; scale bar = 500 µm) and high-magnification insets (right; scale bar = 50 µm). C: quantification obtained from volumetric tissue exploration and analysis was plotted, showing percentages of autofluorescent cells out of the total number of cells. Data are expressed as means ± SE; n = 3 animals per group. *P < 0.05 vs. vehicle control; #P < 0.05 vs. Cp + DMSO using one-way ANOVA followed by a Tukey’s multiple comparisons test.