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. 2021 Dec 15;9:806788. doi: 10.3389/fbioe.2021.806788

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Copyright © 2021 Guan, Wang, Gao, Li, Yan, Ji, Ren, Wang, Zhou, Li and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Purification of recombinant TanA_Lb protein. (A) The purification of TanA_Lb by ion exchange chromatography. (B) The purification of TanA_Lb by size-exclusion chromatography. (C) SDS-PAGE analysis of the expression and purification of His6-TanA_Lb. Lane M: protein molecular weight markers; Lanes 1–2: crude extract; Lane 3: flowthrough; Lane 4: unbound proteins; Lane 5: the sample before elution; Lane 6: fractions eluted from his affinity resin.