Fig. 1.

Glutaredoxin 2c inhibits differentiation of mouse oligodendrocyte progenitor cells into mature oligodendrocytes. A) Primary mouse A2B5⁺ cells differentiate into mature MBP⁺ oligodendrocytes via NG2⁻glia and CNPase⁺ cells. Immunocytochemical analyses were performed after 5 days of differentiation, scale bar: 10 μm. B) Differentiation of A2B5⁺ cells with or without treatment with 2 μM recombinant Grx2c, quantification after immunohistochemistry from 3 independent biological samples. C) qRT-PCR of indicated genes after 5 days of differentiation of A2B5⁺ cells using GAPDH for normalization. D) Representative western blot analyses and quantification of 3 independent experiments after 5 days of differentiation of A2B5⁺ cells. E) Differentiation of NG2⁺ cells with or without treatment with 2 μM recombinant Grx2c, quantification after immunohistochemistry from 3 independent biological samples. F) qRT-PCR of indicated genes after 5 days of differentiation of NG2⁺ cells treated either with control siRNA or Grx2siRNA. Data were normalized to transcripts of GAPDH. *: p < 0.05 (Student's t-test).