Fig. 2.

Glutaredoxin 2c increases migratory capacity of mouse NG2⁺ glia. A) NG2⁺ cells were seeded in a Boyden chamber and incubated with 2 μM Grx2cwt or Grx2cC37S or transfected either with control siRNA or Grx2siRNA. Transmigrated cells were counted. B) Two-photon-microscopy of dorsally migrating oligodendrocyte progenitor cells in Tg(olig2-GFP) zebrafish at 3 dpf with and without morpholino-induced Grx2 knock-down. C) Migrated cells shown in b) were counted in zebrafish 3 dpf comparing Grx2 knock-down or Grx2 overexpression with respective controls. D) Quantified gap closure after scratch assay at indicated time points by NG2⁺ cells treated with 2 μM recombinant Grx2c or not. E) Immunocytochemistry of control and Grx2c-treated NG2⁺ cells after scratch assay at indicated time points. *: p < 0.05; **: p < 0.01; ***: p < 0.001 (Student's t-test).