Fig. 6.

SP-1 is a main target of glutaredoxin 2′s regulation of migration. A) Treatment with 5 and 10 nM of the SP-1 inhibitor mithramycin A abolished the positive effect of Grx2c incubation on GBM-18 cell migration, ***: p < 0.001 (Student's t-test). B) Survival of GBM-18 cells treated with indicated concentrations of mithramycin A was tested using the Cell Titer Blue assay. C) CSPG4 transcripts in GBM-18 cells treated with 10 or 25 nM mithramycin A measured by qRTPCR and normalized to GAPDH transcripts. D) GLRX2 transcripts in the invading areas compared to all other areas of a tumor according to the IVY GAP project. ***: p < 0.001 (ANOVA). E) The Yin and Yang of the (patho-) physiological consequences of Grx2c regulated CSPG4 transcription. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)