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. 2021 Nov 25;55:101406. doi: 10.1016/j.molmet.2021.101406

Figure 1.

Figure 1

Regulation of Glo1 activity via tyrosine 136 phosphorylation.(A) Mutation of Glo1 Y136 to alanine reduces Glo1 activity. Glo1 knockout HeLa cells were used to express 9 different point mutants of Glo1 or the wildtype protein as a control. Glo1 protein levels were quantified by immunoblotting and imaging with a Chemidoc (lower panel) and used to normalize Glo1 activity measurements, to arrive at Glo1 specific activity (upper panel). n = 4, ∗p < 0.05, ∗∗p < 0.01 by mixed-effect ANOVA with Geisser-Greenhouse correction. (B) Mutation of Glo1 Y136 to phenylalanine reduces Glo1 activity. Glo1 protein levels and activity were quantified as in panel A. In all assays, Glo1 activity was normalized by total Glo1 protein. n = 10, ∗∗∗p < 0.001 by two-sided unpaired t-test.