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. 2021 Nov 25;55:101406. doi: 10.1016/j.molmet.2021.101406

Figure 4.

Figure 4

Glucose levels regulate Glo1 Y136 phosphorylation and Glo1 activity in HepG2 and U-937 cells.(A–C) Glo1 Y136 phosphorylation responds to glucose levels in a bimodal fashion, increasing from 0 mM to 5 mM glucose (A) and then decreasing when cells are cultured with a medium containing high glucose concentrations (B–C). Cells were cultured overnight in low (0–2 mM), physiological (5 mM), or hyperglycemic (25 mM) glucose concentrations. n = 4 biological replicates for (A), 5 biological replicates for (B), and 2 biological replicates for (C). Error bars = std. dev. (D–E) Glo1 activity decreases in HepG2 cells (A) or U-937 cells (B) when cells are cultured with a medium containing high glucose concentrations. Cells were treated with normal or high glucose overnight as in B–C, followed by quantification of Glo1 activity. n = 10 biological replicates for D, and 2 biological replicates for E. For all panels, ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001 by two-sided unpaired t-test.