Figure 8.

Mutually antagonistic relationship between canonical and noncanonical autophagy in the exocrine pancreas. (A–F) WT mouse pancreatic acinar cells were transduced with adenovirus bearing mouse GFP-Rab9 (or “mock”) for indicated times and treated (or not) for 1 hour with 100 nmol/L CCK. (G–I) Acinar cells were isolated from Atg5^Δpan and f/WT (control littermate) mice and treated (or not) for 1 hour with 100 nmol/L CCK. (A–D, G–I) IB analysis of indicated proteins. Densitometric band intensities were normalized to endogenous Rab9 (B) or ERK in the same sample and further normalized to those in control, mock-transduced (B–D) or f/WT (H and I) cells. (E and F) Necrosis was measured as the percentage of propidium iodide (PI)-permeable acinar cells. Scale bars, 10 μm. (J) Schematic depicting mutually antagonistic relationship between noncanonical and canonical autophagy in acinar cells. Values are mean ± SEM from 3 to 4 cell preparations for each condition. In (F), each symbol corresponds to a different group of acinar cells (n = 21–32 groups of cells analyzed per condition). ∗P < .05, ∗∗P < .01, ∗∗∗P < .001 vs the same parameter in control (no CCK) cells, mock-transduced (B–F), or f/WT (H and I). ^##P < .01, ^###P < .001 vs CCK-treated mock-transduced (B–F) or f/WT (H and I) cells. ˆP < .05, ˆˆP < .01, ˆˆˆP < .001 vs control (no CCK) cells transduced with AdV-Rab9 (C and F) or f/WT cells (I). ^$$$P < .001 vs the same parameter in 8-hour transduced cells (B). Significance was determined by 1-way ANOVA, followed by Tukey or Holm-Sidak multiple comparisons test.