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Properties of the VIP + ALI epithelial monolayers. (a) TEER was measured across confluent layers of colonic epithelial cells (n = 3), cultured on microporous inserts. Additionally, a microporous insert with no cells (submerged in PBS) was measured as a control. (b) Mucus was collected from monolayers (n = 3 per day) over 2–7 days and the weight percentage of solids was measured. (c) The pH of the apical and basal reservoirs of cell monolayers was measured (n = 9). ∗p < 0.05.
