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(A) 3D histograms plotting single-molecule FRET efficiency (EFRET) distributions of CaM-bound Cy3/Cy5-labeled CLeNOSr versus the dwell times that the molecules stay at the given EFRET. The FRET peaks indicate that three major conformational states exist. The dwell time distributions of the closed state (B), intermediate state (C), and the open state (D).
