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. 2021 Sep 26;13(2):623–642. doi: 10.1016/j.jcmgh.2021.09.012

Figure 2.

Figure 2

IL13 regulates murine organoid cellular composition, promotes growth and survival, and alters Muc6 and Muc5ac expression. (A) Representative organoid photographs on day 9 cultured without (left) and with (right) 5 ng recombinant mouse IL13. Scale bars: 200 μm. (B) Left: Quantification of the mean diameter of organoids cultured without (blue) and with (red) 5 ng recombinant mouse IL13. Each dot represents the mean diameter from 1 independent experiment (N = 10). ∗∗P = 0.002. Right: Quantification of organoid viability. Each dot represents the proportion of viable organoids from 1 independent experiment (N = 5–6). ∗∗∗P = 0.0003. (C) Representative immunofluorescent staining of organoids cultured without (left) or with (right) 5 ng recombinant mouse IL13 on day 7. Top: MUC5AC expression represented by UEA-I (green), anti–E-cadherin (white), and Hoechst (blue). Bottom: MUC6 expression represented by GSII (red). Scale bars: 50 μm. (D) qPCR analysis of gene expression in organoids cultured without (blue) or with (red) 5 ng recombinant mouse IL13 on day 7. Data show fold change expression. Each dot represents 1 independent experiment (N = 3–11). Muc5ac: ∗∗∗P = 0.0003; Muc6: ∗∗∗∗P <0.0001; Tff2: ∗∗∗∗P < 0.0001. (E) Proportion of UEA-I+ cells (left) and GSII+ cells (right). Each dot represents the percentage of positive cells from 1 independent experiment (N = 4). ∗P = 0.033 ∗∗P = 0.002. Significance was determined using a Student t test. Data show means ± SEM.