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. 2021 Sep 26;13(2):623–642. doi: 10.1016/j.jcmgh.2021.09.012

Figure 4.

Figure 4

IL13 acts on gastric epithelial cells to increase organoid size and viability. (A) Summary violin plots showing transcript expression in TxA23 mice of the IL13-receptor subunits Il4ra (top) and Il13ra1 (bottom) on the corpus epithelial cell subsets: neck cells, foveolar cells, chief cells, and parietal cells. Above each plot the cell subset frequency of cells with a transcript expression level >0.1 is labeled. (B) Representative photographs of BALB/c (top) and BALB/cxIl4ra-/- (bottom) organoids cultured with immune cells isolated from TxA23 mice. Photographs are of day 9 organoids cultured without (left) and with (right) 5 ng recombinant human IL13. Scale bars: 200 μm. (C) Left: Quantification of the mean diameter of organoids cultured with (red) 5 ng recombinant human IL13. Each dot represents the mean diameter from 1 independent experiment (N = 3). ∗P = 0.03. Right: Quantification of organoid viability. Each dot represents the proportion of viable organoids from 1 independent experiment (N = 3). ∗∗P = 0.004. Significance was determined using a Student t test. Data show means ± SEM.