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. 2021 Oct 30;120(23):5295–5308. doi: 10.1016/j.bpj.2021.10.033

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(a) Schematic detailing the assembly process of surface-tethered BamA^(L7H9)BCDE/POPC sensor surfaces. (b) QCM-D data following the assembly procedure. (c) PNR data (points) and fits (lines) corresponding to D₂O solution contrast with both spin-up (↑) and spin-down (↓) magnetic contrasts for the functionalized gold substrates before hBamA^(L7H9)BCDE adsorption (blue), after the adsorption of hBamA^(L7H9)BCDE within β-OG micelles (green) and upon reconstitution of hBamA^(L7H9)BCDE within a POPC bilayer (blue). (d) SLD profiles of the complete interface throughout the assembly process of a hBamA^(L7H9)BCDE/POPC proteomembrane, derived from fits to NR data shown in (c and e). SLD profile of the functionalized gold/water interface derived from fits shown in (c). Shaded regions represent 95% confidence intervals determined from MCMC resampling of the experimental data fits.