Figure 4.

(a) PNR data (points) and fits (lines) for a hBamA^(L7H9)BdCDE:POPC membrane adsorbed to a DTSP-ANTA-Cu²⁺ functionalized gold surface, characterized in both spin-up (↑) and spin-down (↓) magnetic contrasts in D₂O (red data), Gold-matched water (gold data), PrMW (green data) and H₂O (blue data) solution isotopic contrasts. (b) SLD profiles (top panel) and component volume fraction profile (bottom panel) corresponding to fits shown in (a). (c) Component volume fraction profile corresponding to fits shown in (a), describing the membrane-containing interfacial region. Shaded regions represent the 95% confidence intervals determined from MCMC resampling of fits to the experimental data. Vertical dashed lines show layer boundaries for the interfacial proteo-bilayer region, overlaid with a schematic representation of a high-resolution model of the BamABCDE complex (Protein Data Bank: 5LJO) situated within a POPC bilayer.