Table 3.
RNAi and Vector Construct Information
| Name | Sequence | Company (catalog #) |
|---|---|---|
| tracrRNA | N/A | Dharmacon (Lafayette, CO, #U-002005-05) |
| KDM6A crRNA | TACTGAATTGCTAGGCAGGG | Dharmacon (Lafayette, CO, #CM-014140-04) |
| scramble siRNA | UGGUUUACAUGUCGACUAA | Dharmacon (Lafayette, CO, #D-001210-05) |
| KDM6A siRNA1 | GAGAGUAAUUCACGAAAGA | Dharmacon (Lafayette, CO, #D-014140-02) |
| KDM6A siRNA2 | CAGCACGAAUUAAGUAUUU | Dharmacon (Lafayette, CO, #D-014140-04) |
| INHBA siRNA | GAACGGGUAUGUGGAGAUA | Dharmacon (Lafayette, CO, #D-011701-02) |
| piLenti-siRNA- scramble-GFP | GGGTGAACTCACGTCAGAA | abm (Richmond, BC, Canada, #LV015-G) |
| piLenti-siINHBA-GFP | ACCTCGGAGATCATCACGTTTGCCGAGTC | abm (Richmond, BC, Canada, #i010997) |
| pGipZ-scramble-GFP | N/A | Open Biosystems (Huntsville, AL, #RHS4346) |
| pGipZ-shKDM6A-GFP | N/A | Open Biosystems (Huntsville, AL, #V2LHS_66490) |
GFP, green fluorescent protein; N/A, not applicable; siRNA, small interfering RNA.