10× DNA annealing buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-HCl (pH8.0) | 400 mM | 2.4227 g |
| NaCl | 500 mM | 1.4611 g |
| MgCl2 | 100 mM | 0.4761 g |
| ddH2O | n/a | n/a |
| Total | n/a | 50 mL |
Store at 4°C for up to 1 year
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