Reagent | Final concentration | Amount |
---|---|---|
10× PBS | 1 × | 5 mL |
MgCl2 (1 M) | 1 mM | 0.05 mL |
KCl (1 M) | 2.5 mM | 0.125 mL |
OptiPrep | n/a | 33.33 mL |
ddH2O | n/a | 11.495 mL |
Total | n/a | 50 mL |
Store at 4°C for up to 1 year
Reagent | Final concentration | Amount |
---|---|---|
10× PBS | 1 × | 5 mL |
MgCl2 (1 M) | 1 mM | 0.05 mL |
KCl (1 M) | 2.5 mM | 0.125 mL |
OptiPrep | n/a | 33.33 mL |
ddH2O | n/a | 11.495 mL |
Total | n/a | 50 mL |
Store at 4°C for up to 1 year