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. 2021 Dec 23;3(1):101058. doi: 10.1016/j.xpro.2021.101058

Figure 10.

Figure 10

Size Exclusion chromatography purification of RfxCas13d

(A) Chromatogram from RfxCas13d (113 kDa) size exclusion chromatography. Absorption at 280 nm vs eluted volume.

(B) SDS-PAGE of input (In – step 54) and elution fractions of main peak (F7 to F23 – step 57) references. Performed in a 10% polyacrylamide gel and run at 200 V for 45 min. Stained with InstantBlue® Coomassie Protein Stain (Abcam) following manufacturer protocol. M is the molecular weight ladder (Precision Plus Protein Standard All Blue – BioRad).