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SDS-PAGE of 3 different (known concentration) dilutions of BSA and 3 different (unknown concentration) dilutions of RfxCas13d (113 kDa)
Performed in a 10% polyacrylamide gel and run at 200 V for 45 min. Stained with InstantBlue® Coomassie Protein Stain (Abcam) following manufacturer protocol. M is the molecular weight ladder (Precision Plus Protein Standard All Blue – BioRad). The calibration curve and final quantification were performed with the Image Lab software (Bio-Rad).
