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. 2021 Dec 23;3(1):101058. doi: 10.1016/j.xpro.2021.101058

Figure 3.

Figure 3

Schematic representation of the fill-in-PCR to generate the DNA template

The Universal Primer contains the T7 promoter followed by 5′GG and the processed direct repeat for RfxCas13d gRNA, meanwhile, the Specific Primer contains the repeat sequence in reverse complement orientation to hybrid with the universal primer and the specific binding sequence of 22 nt. The product of the Fill-in-PCR is the DNA template that contains the T7 promoter with the 2 “GG” (19 nt), the processed direct repeat (30 nt) and the specific binding site sequence (22nt).