Skip to main content
. 2021 Dec 23;3(1):101058. doi: 10.1016/j.xpro.2021.101058

Figure 9.

Figure 9

Affinity chromatography purification of RfxCas13d

(A) Chromatogram from RfxCas13d (113 kDa) Histidine affinity chromatography. Blue line (left): absorption at 280 nm; green line (right): % of elution buffer; both vs eluted volume.

(B) Expansion of the elution peak from previous chromatogram, showing the fractions collected.

(C) SDS-PAGE of input (In – step 49), flow through (Out – step 50), wash (W - step 51) and peak fractions (F4, F5, F5 and F7) references. Performed in a 10% polyacrylamide gel and run at 200 V for 45 min. Stained with InstantBlue® Coomassie Protein Stain (Abcam) following manufacturer protocol. M is the molecular weight ladder (Precision Plus Protein Standard All Blue – BioRad).