| Reagents | Amount |
|---|---|
| gRNA DNA Template (From step 6) | 6.3 μL |
| ATP (100 mM) | 1.8 μL |
| CTP (100 mM) | 1.8 μL |
| GTP (100 mM) | 1.8 μL |
| UTP (100 mM) | 1.8 μL |
| DTT (100 mM) | 2 μL |
| AmpliScribe-T7 Flash 10× – Reaction Buffer | 2 μL |
| RiboGuard RNase Inhibitor | 0.5 μL |
| AmpliScribe T7-Flash Enzyme Solution | 2 μL |
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