TSS 2× Buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| LB broth (LB Lennox) | 20 g/L | 0.2 g |
| Polyethylene glycol 8000 (PEG) | 200 g/L | 2 g |
| Dimethyl sulfoxide (DMSO) | 10% | 1 mL |
| MgSO4·7H2O | 100 mM | 246.5 mg |
| ddH2O | n/a | Up to 10 mL |
| Total | n/a | 10 mL |
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