Reagent | Final concentration | Amount |
---|---|---|
HEPES·KOH 1M pH 7.5 | 50 mM | 25 mL |
KCl | 500 mM | 18.64 g |
DTT | 1 mM | 77.1 mg |
Glycerol | 10% (v/v) | 50 mL |
Imidazole | 10 mM | 0.34 g |
ddH2O | n/a | Up to 500 mL |
Total | n/a | 500 mL |
Reagent | Final concentration | Amount |
---|---|---|
HEPES·KOH 1M pH 7.5 | 50 mM | 25 mL |
KCl | 500 mM | 18.64 g |
DTT | 1 mM | 77.1 mg |
Glycerol | 10% (v/v) | 50 mL |
Imidazole | 10 mM | 0.34 g |
ddH2O | n/a | Up to 500 mL |
Total | n/a | 500 mL |