Elution Buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| HEPES·KOH 1M pH 7.5 | 50 mM | 5 mL |
| KCl | 500 mM | 3.73 g |
| DTT | 1 mM | 15.4 mg |
| Glycerol | 10% (v/v) | 10 mL |
| Imidazole | 500 mM | 3.40 g |
| ddH2O | n/a | Up to 100 mL |
| Total | n/a | 100 mL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.