Reagent | Final concentration | Amount |
---|---|---|
HEPES·KOH 1M pH 7.5 | 50 mM | 25 mL |
KCl | 250 mM | 9.32 g |
DTT | 1 mM | 77.1 mg |
Glycerol | 10% (v/v) | 10 mL |
ddH2O | n/a | Up to 100 mL |
Total | n/a | 500 mL |
Reagent | Final concentration | Amount |
---|---|---|
HEPES·KOH 1M pH 7.5 | 50 mM | 25 mL |
KCl | 250 mM | 9.32 g |
DTT | 1 mM | 77.1 mg |
Glycerol | 10% (v/v) | 10 mL |
ddH2O | n/a | Up to 100 mL |
Total | n/a | 500 mL |