E3 buffer 60× buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| NaCl | 300 mM | 34.8 g |
| KCl | 10.2 mM | 1.6 g |
| CaCl2-2H2O | 20 mM | 5.8 g |
| MgCl2-6H2O | 20 mM | 10 mL |
| ddH2O | n/a | Up to 2 L |
| Total | n/a | 2 L |
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