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. 2021 Dec 23;3(1):101058. doi: 10.1016/j.xpro.2021.101058
Reagents Amount
Universal Primer (100 μM) 2.5 μL
Specific Primer (100 μM) 2.5 μL
dNTPs (40mM) 0.5 μL
Buffer 5× 5 μL
Q5® High-Fidelity DNA Polymerase (New England Biolabs #M0491) 0.25 μL
DNase/RNase free water 14.25 μL
Total 25 μL