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. Author manuscript; available in PMC: 2021 Dec 29.
Published in final edited form as: Diabetologia. 2021 Jul 17;64(10):2279–2291. doi: 10.1007/s00125-021-05501-8

Fig. 1.

Fig. 1

Cellular localisation of PrPC in beta cells of human pancreases based on immunofluorescence analysis, (a, e, i) PrPC was observed to be highly expressed in pancreatic islets of all three islet types [non-diabetic; type 1 diabetic INS(+); type 1 diabetic INS(−)] with low level expression in the exocrine pancreas, (a–d) PrPC expression in non-diabetic donors localises to pancreatic islets and co-localises with beta cells (c). Co-registration of PrPC/INS cells was confirmed using z-stack 3D image reconstruction analysis (d). (e, i) In our analysis of PrPC in type 1 diabetic pancreas, we separated images from donors that contain both INS(+) (e) and INS(−) islets (i). (e)–h) As with non-diabetic donors, type 1 diabetic INS(+) islets show co-registration between PrPC and INS cells (g, h). (i–l) In the absence of insulin [INS(−)], PrPC continues to be expressed in a large number of cells (i, k). Scale bars, 20 αm. INS, insulin; ND, non-diabetic, T1D, type 1 diabetic