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. 2021 Sep 24;7(9):000634. doi: 10.1099/mgen.0.000634

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© 2021 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 2. — Flanking regions 5000 bp upstream of bla _OXA-48 in plasmids from K . pneumoniae isolates. The Tree panel is a neighbour-joining tree reconstructed from Mash distances between complete sequences of plasmids carrying the bla _OXA-48 gene. The second panel indicates the presence/absence of a L/M(pOXA-48)-type plasmid. The Gene Graphical Representation panel schematically represents coding regions in the 5000 bp sequence upstream of the bla _OXA-48 gene, which is shown in red. Other genes are coloured according to the FP, which considers the overall pattern of all 100 bp window clusters up to 2200 bp (the approximate upstream limit of Tn1999). The Flankergram panel shows window clusters of all groups over each 100 bp window between 0 and 5000 bp. The dotted line at 2200 bp indicates the approximate point of upstream divergence between several FPs. The MLST panel shows K. pneumoniae multilocus sequence types, with those occurring once labelled ‘other’. FPs are numbered in ascending order according to abundance in the hybrid assemblies. Data used to make this figure came from the Dutch CPE surveillance and EuSCAPE hybrid assembly datasets.