Table 1.

Comparison of the Immucor and One Lambda protocol^a using beadset C. For the brand names and manufacturers names of the beadset refer to Materials and methods.

mAb tested	Beadset	Loci	Alleles examined	Protocol	MFI median ± SD	MFI range	p value
W6/32	Beadset C	HLA-A	29	Immucor	17,553 ± 1989	13,869–20,885	0.87
				One Lambda	17,146 ± 2242	13,662–21,638
		HLA-B	48	Immucor	19,231 ± 2180	11,482–22,266	0.96
				One Lambda	19,365 ± 2371	10,683–22,887
		HLA-Cw	18	Immucor	11,859 ± 2757	5990–15,660	0.53
				One Lambda	11,364 ± 2979	5336–15,633

^aThe Immucor protocol: 40 μL of bead mixture was incubated with 10 μL of mAbs at the concentration of 50 μg/mL for 30 min at RT.

The One Lambda protocol: 2 μL of bead mixture was incubated with 20 μL of mAbs at the concentration of 10 μg/mL for 30 min at RT.

Note that the stock concentration of the bead solution differ between beadsets A and C: Beads of Beadset C are diluted 8 folds compared to Beadset A;