Table 2.

Comparison of the reactivity of mouse mAbs W6/32, HC-10 and TFL-006 with HLA-I coated three different beadsets A, B and C. For the commercial sources and the brand names of the beadsets refer to Materials and methods.

mAbs tested	Loci	n	Beadset	Median MFI ± SD	MFi range	p value vs Beadset C
W6/32	HLA-A	27	A	21,664 ± 950	19,008–22,513	< 0.0001
			B	17,159 ± 1978	11,450–19,860	< 0.0754
			C	17,308 ± 2240	13,663–21,638
	HLA-B	44	A	21,811 ± 1568	16,084–23,072	< 0.0001
			B	17,481 ± 3178	10,433–21,406	0.0002
			C	19,635 ± 2392	10,863–22,887
	HLA-Cw	13	A	18,797 ± 3768	11,436–23,742	0.0015
			B	13,258 ± 4196	4918–18,639	0.75
			C	10,825 ± 3095	5336–15,299
HC-10	HLA-A	10	A	10,645 ± 4274	5038–17,797	0.0051
			B	3732 ± 1882	1488–7112	0.0051
			C	426 ± 256	97–905
	HLA-B	42	A	14,066 ± 3859	3057–19,455	< 0.0001
			B	1202 ± 1296	370–6922	0.028
			C	838 ± 1253	67–5736
	HLA-Cw	13	A	18,322 ± 3115	12,096–21,774	0.0015
			B	732 ± 686	455–2650	0.13
			C	791 ± 423	231–1627
TFL-006	HLA-A	27	A	1172 ± 1312	242–6907	< 0.0001
			B	231 ± 338	80–1468	< 0.0001
			C	1 ± 1	0–5
	HLA-B	44	A	2257 ± 1158	205–4806	< 0.0001
			B	13 ± 45	4–225	< 0.0001
			C	0 ± 1	0–6
	HLA-Cw	13	A	3702 ± 1932	1852–7733	0.0015
			B	18 ± 18	9–72	0.0019
			C	2 ± 5	0–15