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. 2021 Dec 15;9:737621. doi: 10.3389/fcell.2021.737621

FIGURE 1.

FIGURE 1

Fluorescence and TEM micrographs of HeLa cells after IRF protocol. (A) Cells fixed with 4% PFA and scraped, then processed with 0.1% uranyl acetate; (B) Trypsinized cells freeze-substituted with 0.1% uranyl acetate; (C, D) Trypsinized cells freeze-substituted and processed with 0.2 and 0.5% uranyl acetate, respectively. Immunofluorescence light microscopy imaging with a Nikon TiE inverted C2 confocal with Plan Apo VC 20x dry lens (left column), TEM at low and high magnification (resp. middle and right column); images in the right column correspond to boxed regions. Note the preservation of fluorescence in conditions (A–C) and the best ultrastructure in (C–D). IRF, in-resin fluorescence; PFA, paraformaldehyde; TEM, transmission electron microscopy; UrAc, uranyl acetate. Scale bars: left column = 20 μm; middle = 2 μm; right = 1 µm.