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. 2021 Dec 15;9:737621. doi: 10.3389/fcell.2021.737621

FIGURE 6.

FIGURE 6

Image quality as function of section thickness (vertical) and accelerating voltage (horizontal). All images represent trypsinized cells freeze-substituted with 0.2% UrAc and sectioned at 300 nm (A), 200 nm (B) and 50 nm (C). All images are acquired by SEM and BSE-detector. The thicker the section, the higher the tolerated accelerating voltage is before quality breaks down. WD A 1.5 kV = 5 mm; A 2 kV = 4.6 mm; A 3 kV = 4.3 mm; B 1.5 kV = 5.1 mm; B 2 kV = 4.7 mm; B 3 kV = 4.4 mm; C 1.5 kV = 5.2 mm; C 2 kV = 4.8 mm; C 3 kV = 4.5 mm. Abbreviations: BSE, backscattered electrons (Gatan OnPoint detector); SEM, scanning electron microscope; UrAc, uranyl acetate; WD, working distance. Scale bars: 1 µm.